Effect of different intensities of calorie restriction on liver and muscle mitochondrial oxidative stress

The aim of the present study was to evaluate the effect of different levels of calorie restriction (CR) on liver and muscle mitochondrial oxidant production and antioxidant enzymes activity in adult rats. Male Sprague Dawley rats (n=28) were subjected to 25-75% CR for 15 days. H2O2 production was decreased in muscle mitochondria of 25-50% CR group. H2O2 production decreased in liver mitochondria of 25% CR group. The protein carbonyl content decreased in liver and muscle mitochondria of 25-50 % CR groups. TBARS levels were decreased in liver and muscle of (25-50%) CR groups. Superoxide dismutase and glutathione peroxidase activity was increased in liver mitochondria of 25% and 50% CR groups. Catalase activity was increased in liver and muscle of 25-50% CR groups. The 4-Hydroxy-2neonal modified proteins increased in muscle of 75% CR rats. Results of the present study demonstrate that short term CR of 25-50% reduces the oxidative stress levels in muscle and liver mitochondria.